VANASYL
The Vanasyl Sampling System: The Vanasyl Sampling Valve and Vanasyl Sampling Bags.
 
The Vanasyl Sampling System

In biopharmaceutical production, specifically in liquid form, such as injectibles, both in processes using mammalian cells and microbial cells, the processes that needs to take place successfully in a bioreactor, fermentation and cell growth, are critical for large scale manufacturing. To ensure that the cells grow properly in the bioreactor, samples are periodically taken, to measure delivered oxygen, oxygen, carbon dioxide, glucose levels and other parameters that can either assist or inhibit cell growth. Based on the sample results, media feed decisions are made. If the sample readings are not true representations of what it is in the bioreactor, incorrect media feed decisions will be made which could result in improper feedings and limitation of cell growth or even worse, premature cell death. Batches are often disposed of, costing companies thousands of dollars.

The VANASYL SAMPLING SYSTEM can prevent this.

How the VANASYL sampling device works: It is a stainless-steel valve, with a 3-position handle that either is set to steam, off or sample. Its seals are carbon-filled PTFE so steam can run through the valve continuously for millions of cycles. It’s made with high precision engineering; sits flush to the bioreactors, allows no dead leg, no area where media could sit and contaminate the previous sample. Shutting off the steam is done by the valve itself which saves time and device can simply be switched to the steam position after sampling. Multiple samples can be taken. The device is self-cleaning; inner volume is small so the small amount of condensate that accumulates after the steam is shut, once the valve is back to the steam position, the steam pushes the condensate, flushing the device clean.

 The connection from the device to the sampling receptacle is a Clave® fitting.  It is a fitting used in hospitals for IV; it is aseptic and closed; liquid only passes through deep within the fitting; at not time at all does the surfaces come into contact with the fluid path. This keeps the sample operation aseptic. Not only is the sampling operation aseptic and closed, but the sample stays sterile in the bag. Periodic samples can be taken out of bag with a sterile syringe and immediately tested in the gas analyzer and other testing instrumentation. There is no need for a safety cabinet or to transfer the sample from the bag into a bottle. This allows faster sampling, thus more accurate. The sampling bag/receptacle not only is robust with very low extractables, but it is also chemically, physically and biologically compatible with media fluid and also freezable to 150°C so samples can be withdrawn, remaining media can be frozen to be sampled at a later date. The only maintenance is simply changing the Ingold O-ring after every batch, and the inlet/outlet O-rings every six months. The valve should never be disassembled.

 USE ONLY THE VANASYL SYSTEM FOR YOUR FERMENTATION PROCESSES!



CRITICAL DOCUMENTATION